| Seniority | Member Name | Party | Senate | House |
|---|---|---|---|---|
| 1 | L. Louise Lucas | Democrat | 1992- | |
| 2 | R. Creigh Deeds | Democrat | 2001- | 1992-2001 |
| 3 | Mark D. Obenshain | Republican | 2004- | |
| 4 | Mamie E. Locke | Democrat | 2004- | |
| 5 | Ryan T. McDougle | Republican | 2006- | 2002-2006 |
| 6 | Richard H. Stuart | Republican | 2008- | |
| 7 | David W. Marsden | Democrat | 2010- | 2006-2010 |
| 8 | William M. Stanley, Jr. | Republican | 2011- | |
| 9 | Adam P. Ebbin | Democrat | 2012- | 2004-2012 |
| 10 | Bryce E. Reeves | Republican | 2012- | |
| 11 | Barbara A. Favola | Democrat | 2012- | |
| 12 | Scott A. Surovell | Democrat | 2016- | 2010-2016 |
| 13 | Bill DeSteph | Republican | 2016- | 2014-2016 |
| 14 | Jeremy S. McPike | Democrat | 2016- | |
| 15 | David R. Suetterlein | Republican | 2016- | |
| 16 | Mark J. Peake | Republican | 2017- | |
| 17 | Jennifer B. Boysko | Democrat | 2019- | 2016-2019 |
| 18 | Todd E. Pillion | Republican | 2020- | 2014-2020 |
| 19 | Ghazala F. Hashmi | Democrat | 2020- | |
| 20 | T. Travis Hackworth | Republican | 2021- | |
| 21 | Aaron R. Rouse | Democrat | 2023- | |
| 22 | Lamont Bagby | Democrat | 2023- | 2015-2023 |
| 23 | Glen H. Sturtevant, Jr. | Republican | 2016-2020, 2024- | |
| 24 | Christopher T. Head | Republican | 2024- | 2012-2024 |
| 25 | Emily M. Jordan | Republican | 2024- | 2018–2024 |
| 26 | Lashrecse D. Aird | Democrat | 2024- | 2016–2022 |
| 27 | Schuyler T. VanValkenburg | Democrat | 2024- | 2018-2024 |
| 28 | Danica A. Roem | Democrat | 2024- | 2018–2024 |
| 29 | Angelia Williams Graves | Democrat | 2024- | 2021-2024 |
| 30 | Jennifer D. Carroll Foy | Democrat | 2024- | 2018–2020 |
| 31 | Tara A. Durant | Republican | 2024- | 2022–2024 |
| 32 | Russet W. Perry | Democrat | 2024- | |
| 33 | J.D. "Danny" Diggs | Republican | 2024- | |
| 34 | Stella G. Pekarsky | Democrat | 2024- | |
| 35 | Timmy French | Republican | 2024- | |
| 36 | Saddam Azlan Salim | Democrat | 2024- | |
| 37 | Christie New Craig | Republican | 2024- | |
| 38 | Tammy Brankley Mulchi | Republican | 2024- | |
| 39 | Kannan Srinivasan | Democrat | 2025- | 2024-2025 |
| 40 | Luther Henry Cifers, III | Republican | 2025- |
Seniority is based upon longest continuous service in the Senate. However, if a Senator has previous interrupted service in the Senate, then the beginning date of such previous Senate service shall qualify the Senator for seniority before those Senators elected at the same time not having previous service in the Senate, and if a Senator has previous service in the House of Delegates then the beginning date of such House service shall qualify the Senator to seniority before those Senators elected to the Senate at the same time not having previous service in the House of Delegates.
Senators elected at the same time without previous service in the Senate or House of Delegates shall have their seniority determined by a public drawing of lots, conducted by the Clerk of the Senate, to which all Senators involved shall be invited to attend.